Coding

Part:BBa_K1476001:Design

Designed by: Matthew Smith   Group: iGEM14_Reading   (2014-10-09)


PilA1 from Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed to contained regions homologous to the pilA1 gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to pilA1: the first region is homologous to the first 200 bases of pilA1, the second region to the last 200 bases of the pilT1 gene. The centre of the part contains the Pcpc560 super-strong promoter, the PilA1 gene and a kanamycin resistance gene, which become inserted in the middle of the chromosomal pilT1 gene during recombination.



Source

Organism: Cyanobacteria Genus and Strain: Synechocystis sp. PCC 6803 Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/sll1694 Important genotype features: none

References